Chromosome Analysis Using Spectral Karyotyping (SKY)
نویسندگان
چکیده
منابع مشابه
Spectral karyotyping (SKY)
Published: 13/10/2010 Received: 02/02/2010 ecancer 2010, 4:181 DOI: 10.3332/ecancer.2010.181 Copyright: © the authors; licensee ecancermedicalscience. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the origin...
متن کاملErratum: Spectral Karyotyping (SKY)
Copyright: © the authors; licensee ecancermedicalscience. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
متن کاملThe clinical application of spectral karyotyping (SKY) in the analysis of prenatally diagnosed extra structurally abnormal chromosomes (ESACs).
OBJECTIVE The prenatal detection of de novo extra structurally abnormal chromosomes (ESACs) presents a challenge because the associated risk for congenital anomaly ranges from 100% to practically none, depending on the chromosomal origin. Despite the use of standard cytogenetic techniques and even fluorescence in situ hybridization (FISH), the origin of some ESACs often remains elusive. Spectra...
متن کاملMultivariate chromosome analysis and complete karyotyping using dual labeling and fluorescence digital imaging microscopy.
The combination of multiple dye-DNA interactions, a fluorescence digital imaging system with a scientific CCD camera, and multivariate image analysis allows the rapid karyotyping of fluorescent human metaphase chromosome spreads. Chromosomes are stained with the bisbenzimidazole dye Hoechst 33342 and chromomycin A3, a dye pair used frequently in bivariate flow analysis and sorting of metaphase ...
متن کاملLimitations of chromosome classification by multicolor karyotyping.
Multicolor karyotyping technologies, such as spectral karyotyping (SKY) (Schröck et al.1996; Liyanage et al. 1996) and multiplex (M-) FISH (Speicher et al. 1996), have proved to be extremely useful in prenatal, postnatal, and cancer cytogenetics. However, these technologies have inherent limitations that, in certain situations, may result in chromosomal misclassification. In this report, we pre...
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ژورنال
عنوان ژورنال: Cell Biochemistry and Biophysics
سال: 2011
ISSN: 1085-9195,1559-0283
DOI: 10.1007/s12013-011-9285-2